The extracellular matrix (ECM) is a complex meshwork of cross-linked proteins providing
both biophysical and biochemical cues that are important regulators of cell proliferation,
survival, differentiation, and migration. We present here a proteomic strategy developed to
characterize the in vivo ECM composition of normal tissues and tumors using enrichment of
protein extracts for ECM components and subsequent analysis by mass spectrometry. In
parallel, we have developed a bioinformatic approach to predict the in silico" matrisome" …