[HTML][HTML] Characterization of recombinant human prolyl 3-hydroxylase isoenzyme 2, an enzyme modifying the basement membrane collagen IV
P Tiainen, A Pasanen, R Sormunen… - Journal of biological …, 2008 - ASBMB
The single 3-hydroxyproline residue in the collagen I polypeptides is essential for proper
fibril formation and bone development as its deficiency leads to recessive osteogenesis
imperfecta. The vertebrate prolyl 3-hydroxylase (P3H) family consists of three members,
P3H1 being responsible for the hydroxylation of collagen I. We expressed human P3H2 as
an active recombinant protein in insect cells. Most of the recombinant polypeptide was
insoluble, but small amounts were also present in the soluble fraction. P3H1 forms a …
fibril formation and bone development as its deficiency leads to recessive osteogenesis
imperfecta. The vertebrate prolyl 3-hydroxylase (P3H) family consists of three members,
P3H1 being responsible for the hydroxylation of collagen I. We expressed human P3H2 as
an active recombinant protein in insect cells. Most of the recombinant polypeptide was
insoluble, but small amounts were also present in the soluble fraction. P3H1 forms a …