Background Mast cells (MCs) are best known as key effector cells of allergic reactions, but
they also play an important role in host defense against pathogens. Despite increasing
evidence for a critical effect of MCs on adaptive immunity, the underlying mechanisms are
poorly understood. Objective Here we monitored MC intercellular communication with
dendritic cells (DCs), MC activation, and degranulation and tracked the fate of exocytosed
mast cell granules (MCGs) during skin inflammation. Methods Using a strategy to stain …