Ultrastructural morphometry of the pancreatic β-cell

PM Dean - Diabetologia, 1973 - Springer
PM Dean
Diabetologia, 1973Springer
Standard stereological methods have been used to determine the morphometric parameters
of organelles contained in the β-cella of mouse pancreas. Sectioned material from ten islets
was analysed at three levels of magnification in the electron microscope. Quantitative data
was obtained about the volume number and surface area of the various cytological
components and was expressed as density per cm 3 of tissue and as absolute dimensions
for the average β-cell. The organelles studied were the nucleus, cytoplasmic ground …
Summary
Standard stereological methods have been used to determine the morphometric parameters of organelles contained in theβ- cella of mouse pancreas. Sectioned material from ten islets was analysed at three levels of magnification in the electron microscope. Quantitative data was obtained about the volume number and surface area of the various cytological components and was expressed as density per cm3 of tissue and as absolute dimensions for the averageβ-cell. The organelles studied were the nucleus, cytoplasmic ground substance, rough and smooth endoplasmic reticulum, the ribosomes, mitochondria,β-granules and microtubules.
Springer