[HTML][HTML] The structural basis for monoclonal antibody 5D2 binding to the tryptophan-rich loop of lipoprotein lipase
JG Luz, AP Beigneux, DAK Asamoto, C He… - Journal of lipid …, 2020 - ASBMB
For three decades, the LPL–specific monoclonal antibody 5D2 has been used to investigate
LPL structure/function and intravascular lipolysis. 5D2 has been used to measure LPL
levels, block the triglyceride hydrolase activity of LPL, and prevent the propensity of
concentrated LPL preparations to form homodimers. Two early studies on the location of the
5D2 epitope reached conflicting conclusions, but the more convincing report suggested that
5D2 binds to a tryptophan (Trp)-rich loop in the carboxyl terminus of LPL. The same loop …
LPL structure/function and intravascular lipolysis. 5D2 has been used to measure LPL
levels, block the triglyceride hydrolase activity of LPL, and prevent the propensity of
concentrated LPL preparations to form homodimers. Two early studies on the location of the
5D2 epitope reached conflicting conclusions, but the more convincing report suggested that
5D2 binds to a tryptophan (Trp)-rich loop in the carboxyl terminus of LPL. The same loop …