[HTML][HTML] Heparan Sulfate Proteoglycans Are Primarily Responsible for the Maintenance of Enzyme Activity, Binding, and Degradation of Lipoprotein Lipase in Chinese …
DE Berryman, A Bensadoun - Journal of Biological Chemistry, 1995 - ASBMB
Various aspects of lipoprotein lipase (LPL) metabolism, including cell surface binding,
degradation, and enzymatic activity, were compared between Chinese hamster ovary (CHO)
cells and two distinct proteoglycan-deficient CHO cell lines. The contribution of low density
lipoprotein receptor-related protein in binding LPL was also analyzed by the use of a 39-kDa
receptor-associated protein expressed as a glutathione S-transferase fusion protein (GST-
RAP). Equilibrium binding data with 125 I-LPL revealed the presence of a class of high …
degradation, and enzymatic activity, were compared between Chinese hamster ovary (CHO)
cells and two distinct proteoglycan-deficient CHO cell lines. The contribution of low density
lipoprotein receptor-related protein in binding LPL was also analyzed by the use of a 39-kDa
receptor-associated protein expressed as a glutathione S-transferase fusion protein (GST-
RAP). Equilibrium binding data with 125 I-LPL revealed the presence of a class of high …