[HTML][HTML] A sandwich enzyme-linked immunosorbent assay for human plasma apolipoprotein AV concentration
M Ishihara, T Kujiraoka, T Iwasaki, M Nagano… - Journal of lipid …, 2005 - ASBMB
Apolipoprotein AV (apoA-V) is a recently discovered apolipoprotein that appears to have a
role in plasma triglyceride (TG) transport. We have developed an ELISA for apoA-V using
monoclonal antibodies that has a lower limit of detection of 0.3 ng/ml and linearity up to 20
ng/ml. The ELISA was then used to quantify plasma apoA-V in 196 healthy subjects and 106
patients with insulin-resistant diabetes mellitus. In the healthy subjects, total apoA-V
concentration was 179.2±74.8 ng/ml, and it was greater in females than in males (P< 0.005) …
role in plasma triglyceride (TG) transport. We have developed an ELISA for apoA-V using
monoclonal antibodies that has a lower limit of detection of 0.3 ng/ml and linearity up to 20
ng/ml. The ELISA was then used to quantify plasma apoA-V in 196 healthy subjects and 106
patients with insulin-resistant diabetes mellitus. In the healthy subjects, total apoA-V
concentration was 179.2±74.8 ng/ml, and it was greater in females than in males (P< 0.005) …