Synthesis and transfer of amylase in pigeon pancreatic microsomes
CM Redman, P Siekevitz, GE Palade - Journal of Biological Chemistry, 1966 - ASBMB
A pigeon pancreas microsomal system supplemented with microsomal supernatant fluid or
pH 5 enzyme incorporates radioactive amino acids into purified amylase. Several
parameters of the incorporation system are presented. Tryptic digestion of the isolated
radioactive amylase shows that the amino acids are incorporated into peptide linkage within
the protein, as in amylase synthesized in vivo. The radioactive amylase is first found on the
attached ribosomes and with time is transferred to the deoxycholate-soluble microsomal …
pH 5 enzyme incorporates radioactive amino acids into purified amylase. Several
parameters of the incorporation system are presented. Tryptic digestion of the isolated
radioactive amylase shows that the amino acids are incorporated into peptide linkage within
the protein, as in amylase synthesized in vivo. The radioactive amylase is first found on the
attached ribosomes and with time is transferred to the deoxycholate-soluble microsomal …
