[HTML][HTML] Direct Measurements of Oscillatory Glycolysis in Pancreatic Islet β-Cells Using Novel Fluorescence Resonance Energy Transfer (FRET) Biosensors for …
Pulses of insulin released from pancreatic β-cells maintain blood glucose in a narrow range,
although the source of these pulses is unclear. We and others have proposed that positive
feedback mediated by the glycolytic enzyme phosphofructokinase-1 (PFK1) enables β-cells
to generate metabolic oscillations via autocatalytic activation by its product fructose 1, 6-
bisphosphate (FBP). Although much indirect evidence has accumulated in favor of this
hypothesis, a direct measurement of oscillating glycolytic intermediates has been lacking. To …
although the source of these pulses is unclear. We and others have proposed that positive
feedback mediated by the glycolytic enzyme phosphofructokinase-1 (PFK1) enables β-cells
to generate metabolic oscillations via autocatalytic activation by its product fructose 1, 6-
bisphosphate (FBP). Although much indirect evidence has accumulated in favor of this
hypothesis, a direct measurement of oscillating glycolytic intermediates has been lacking. To …