SUMO ligase activity of vertebrate Mms21/Nse2 is required for efficient DNA repair but not for Smc5/6 complex stability
M Kliszczak, AK Stephan, AM Flanagan, CG Morrison - DNA repair, 2012 - Elsevier
Nse2/Mms21 is an E3 SUMO ligase component of the Smc5/6 complex, which plays multiple
roles in maintaining genome stability. To study the functions of the vertebrate Nse2
orthologue, we generated Nse2-deficient chicken DT40 cells. Nse2 was dispensable for
DT40 cell viability and required for efficient repair of bulky DNA lesions, although Nse2-
deficient cells showed normal sensitivity to ionising radiation-induced DNA damage.
Homologous recombination activities were reduced in Nse2−/−/− cells. Nse2 deficiency …
roles in maintaining genome stability. To study the functions of the vertebrate Nse2
orthologue, we generated Nse2-deficient chicken DT40 cells. Nse2 was dispensable for
DT40 cell viability and required for efficient repair of bulky DNA lesions, although Nse2-
deficient cells showed normal sensitivity to ionising radiation-induced DNA damage.
Homologous recombination activities were reduced in Nse2−/−/− cells. Nse2 deficiency …