[HTML][HTML] Real-time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein
T Nakanishi, M Ikawa, S Yamada, M Parvinen, T Baba… - FEBS letters, 1999 - Elsevier
FEBS letters, 1999•Elsevier
We produced transgenic mouse lines that accumulate mutated green fluorescent protein
(EGFP) in sperm acrosome, a membrane limited organelle overlying the nucleus. The sperm
showed normal fertilizing ability and the integrity of their acrosome was easily examined in a
non-invasive manner by tracing the GFP in individual 'live'sperm with fluorescent
microscopy. The time required for the dispersal of acrosomal contents was demonstrated to
be approximately 3 s after the onset of acrosome reaction.
(EGFP) in sperm acrosome, a membrane limited organelle overlying the nucleus. The sperm
showed normal fertilizing ability and the integrity of their acrosome was easily examined in a
non-invasive manner by tracing the GFP in individual 'live'sperm with fluorescent
microscopy. The time required for the dispersal of acrosomal contents was demonstrated to
be approximately 3 s after the onset of acrosome reaction.
We produced transgenic mouse lines that accumulate mutated green fluorescent protein (EGFP) in sperm acrosome, a membrane limited organelle overlying the nucleus. The sperm showed normal fertilizing ability and the integrity of their acrosome was easily examined in a non-invasive manner by tracing the GFP in individual ‘live’ sperm with fluorescent microscopy. The time required for the dispersal of acrosomal contents was demonstrated to be approximately 3 s after the onset of acrosome reaction.
Elsevier
