Methods for evaluating the acrosomal status of mammalian sperm

NL Cross, S Meizel - Biology of reproduction, 1989 - academic.oup.com
NL Cross, S Meizel
Biology of reproduction, 1989academic.oup.com
A full understanding of the acrosome reaction is central to understanding sperm function.
Acrosomal status can be determined on living, motile sperm in only a few mammalian
species. For other species, many light microscopic methods have been developed, including
colored stains for bright-field microscopy, and probes for fluorescence microscopy. We
review the existing methods and the criteria that should be considered in the choice of an
assay.
Abstract
A full understanding of the acrosome reaction is central to understanding sperm function. Acrosomal status can be determined on living, motile sperm in only a few mammalian species. For other species, many light microscopic methods have been developed, including colored stains for bright-field microscopy, and probes for fluorescence microscopy. We review the existing methods and the criteria that should be considered in the choice of an assay.
Oxford University Press