Human blastocysts grown in culture

PC Steptoe, RG Edwards, JM Purdy - Nature, 1971 - nature.com
PC Steptoe, RG Edwards, JM Purdy
Nature, 1971nature.com
WE have already described the culture of cleaving human embryos to the sixteen celled
stage1, and we now wish to give details of a few embryos that have developed much further,
including two that reached fully developed blastocysts. Methods were similar to those
described before. Preovulatory oocytes recovered by laparoscopy2 were fertilized in
Bavister's medium3, and transferred after 12–15 h into Ham's F 10 supplemented with
human or foetal calf serum. Preliminary details of this work have been presented …
Abstract
WE have already described the culture of cleaving human embryos to the sixteen celled stage1, and we now wish to give details of a few embryos that have developed much further, including two that reached fully developed blastocysts. Methods were similar to those described before. Preovulatory oocytes recovered by laparoscopy2 were fertilized in Bavister's medium3, and transferred after 12–15 h into Ham's F 10 supplemented with human or foetal calf serum. Preliminary details of this work have been presented elsewhere4.
nature.com